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Ngwa Nwepụta Acid Nucleic (A01) Eserese
  • Ngwa Nwepụta Acid Nucleic (A01)
  • Ngwa Nwepụta Acid Nucleic (A01)

Ngwa Nwepụta Acid Nucleic (A01)

Nkọwa dị mkpirikpi:

Ngwa ahụ na-eji bead magnetik nke nwere ike jikọta kpọmkwem na acid nucleic, yana sistemụ nchekwa pụrụ iche.Ọ na-adabara na mmịpụta nucleic acid, ịba ụba, na ịdị ọcha nke sel exfoliated cervical, ụdị mmamịrị, na mkpụrụ ndụ omenala.Enwere ike itinye acid nucleic dị ọcha na PCR, RT-PCR, PCR, usoro na ule ndị ọzọ.Ndị na-arụ ọrụ kwesịrị inwe ọzụzụ ọkachamara n'ịchọpụta ihe ndụ ndụ ma tozuo maka ọrụ nnwale dị mkpa.Ụlọ nyocha ahụ kwesịrị inwe ezigbo nchekwa nchekwa ndu yana usoro nchebe.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ nchọpụta

Mgbe ahapụchara DNA genomic site na kewaa mkpụrụ ndụ na ihe nchekwa lysis, bead magnet nwere ike họrọ ijikọ na DNA genomic dị na nlele.Enwere ike iwepụ ọnụ ọgụgụ dị nta nke adịghị ọcha nke eriri magnetik na-etinye n'ime ya site na ihe nchekwa ihe.Na TE, bead magnetik nwere ike ịhapụ DNA boundgenome, nweta DNA genome dị elu.Usoro a dị mfe na ngwa ngwa na àgwà DNA amịpụtara dị elu, nke nwere ike izute ihe achọrọ maka nchọpụta DNA methylation.Ka ọ dị ugbu a, ngwa mmịpụta nke dabere na bead magnet nwere ike dakọtara na mmịpụta nucleic acid na-akpaghị aka, na-ezute ọrụ mmịpụta nucleic acid dị elu.

Isi ihe nke reagent

E gosipụtara akụkụ ndị a na tebụl 1:

Tebụl 1 Ngwa Reagent na Nbudata

Aha akụrụngwa

Isi ihe

Nha (48)

Nha (200)

1. Lysis ngwọta

Guanidine hydrochloride, Tris

11 ml / karama

44 ml / karama

2. Ngwọta nhicha A

NaCl, Tris

11 ml / karama

44 ml / karama

3. Ngwọta nhicha B

NaCl, Tris

13 ml / karama

26.5mL / karama * 2

4. Eluent

Tris, EDTA

12 ml / karama

44 ml / karama

5. Protease K ngwọta

Protease K

1.1ml / iberibe

4.4ml / iberibe

6. Nkwusioru bead magnetik 1

Igwe igwe magnetik

1.1ml / iberibe

4.4ml / iberibe

7. Ntuziaka iji wepụ nucleic acid reagents

 

1 oyiri

1 oyiri

Ngwa ndị achọrọ na mmịpụta nucleic acid, mana etinyeghị ya na ngwa:

1. Reagent: ethanol anhydrous, isopropanol na PBS;

2. Ihe eji eme ihe: 50ml centrifuge tube na 1.5ml EP tube;

3. Akụrụngwa: Kettle bath mmiri, pipettor, magnetik shelf, centrifuge, 96-oghere miri efere (akpaka), akpaka nucleic acid mmịpụta akụrụngwa (akpaka).

Ozi bụ isi

Ihe atụ chọrọ
1. A ga-ezere mmetọ obe n'etiti ihe nlele na nchịkọta nlele na nchekwa.
2. A ga-emecha nchọpụta ahụ n'okpuru nchekwa ụbọchị 7 nke ọnọdụ okpomọkụ mgbe nchịkọta nke cervical exfoliated cell sample (anaghị edozi).A ga-emecha nchọpụta ahụ n'okpuru nchekwa ụbọchị 30 nke ọnọdụ okpomọkụ mgbe nchịkọta nke mmamịrị;A ga-emecha nchọta ahụ n'oge na-achịkọta ihe nlele cell omenala.

Nkọwa ụgbọ ala:200 pcs / igbe, 48 pcs / igbe.

Ọnọdụ nchekwa:2-30 ℃

Oge nkwado:Ọnwa iri na abụọ

Ngwa ọdabara:Tianlong NP968-C nucleic acid mmịpụta ngwá ọrụ, Tiangen TGuide S96 nucleic acid mmịpụta ngwá ọrụ, GENE DIAN EB-1000 nucleic acid mmịpụta ngwá.

Asambodo ndekọ ngwaọrụ ahụike Mba/ngwaahịa chọrọ teknụzụ Mba.: HJXB Nọmba 20210099.

Ụbọchị nnabata na mgbanwe nke ntuziaka:
Ụbọchị nkwado: Nov. 18, 2021

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