TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa urothelial
NDỤMỌDỤ NKE EKPERE
Nkenkenke
Ekwenyere ihe karịrị ihe nlele ụlọ ọgwụ 3500 n'ime ọmụmụ ihe n'ihu ọha, ngwaahịa a nwere nkọwapụta nke 92.7% yana nghọta nke 82.1%.
Dị mma
Teknụzụ nchọpụta mbụ Me-qPCR methylation nwere ike mechaa n'otu nzọụkwụ n'ime awa 3 na-enweghị mgbanwe bisulfite.
Na-adịghị emerụ ahụ
Naanị 30 ml nke ihe nlele mmamịrị ka achọrọ iji chọpụta ụdị ọrịa kansa atọ, gụnyere ọrịa kansa pelvis, ọrịa urethra, kansa eriri afọ n'otu oge.
Ngwa ndapụta
Nchọpụta ihe enyemaka
Ndị mmadụ na-ata ahụhụ site na hematuria na-enweghị mgbu / nke a na-enyo enyo na ha nwere urothelial (cancer uretral / renal pelvis cancer)
Ntụle Ihe ize ndụ Ọrịa Cancer
Ịwa ahụ/ chemotherapy-chọrọ ndị bi na urothelial carcinoma;
Nleba anya ugboro ugboro
Ndị mmadụ na-arụ ọrụ nke ọma nwere carcinoma urothelial
Ezubere iji
A na-eji ngwa a maka nchọpụta qualitative in vitro nke hypermethylation nke Urothelial Carcinoma(UC) gene na urothelial specimens.Nsonaazụ dị mma na-egosi mmụba dị ukwuu nke UC, nke chọrọ nyocha ọzọ cystoscope na/ma ọ bụ nyocha histopathological.N'ụzọ megidere nke ahụ, nsonaazụ ule na-adịghị mma na-egosi na ihe ize ndụ nke UC dị ala, mana enweghị ike iwepụ ihe ize ndụ ahụ kpamkpam.Nchọpụta ikpeazụ kwesịrị ịdabere na cystoscope na / ma ọ bụ nsonaazụ histopathological.
Ụkpụrụ Nchọpụta
Ngwa a nwere nucleic acid mmịpụta reagent na PCR reagent nchọpụta.A na-ewepụta acid nucleic site na usoro nke dabere na bead magnet.Ngwa a dabere na ụkpụrụ nke fluorescence quantitative PCR usoro, na-eji methylation-kpọmkwem ozugbo PCR mmeghachi omume iji nyochaa DNA ndebiri, na n'otu oge chọpụta saịtị CpG nke UC gene na àgwà akara akara n'ime ime akwụkwọ ntụaka mkpụrụ ndụ iberibe G1 na G2.A na-agbakọ ọkwa methylation nke UC gene, nke akpọrọ dị ka uru m, dabere na uru UC gene methylated DNA amplification Ct na uru Ct nke ntụaka ahụ.A na-ekpebi UC gene hypermethylation nti ma ọ bụ ọnọdụ adịghị mma dịka uru m siri dị.
Ngwa nyocha Methylation DNA (qPCR) maka ọrịa kansa urothelial
| Ngwa ụlọ ọgwụ | Nchọpụta ihe enyemaka ụlọ ọgwụ nke ọrịa cancer urothelial;nyocha arụmọrụ ịwa ahụ / chemotheray;nleba anya nlọghachi azụ nke arụ ọrụ |
| Nchọpụta mkpụrụ ndụ ihe nketa | UC |
| Ụdị ihe atụ | Urine exfoliated cell sample (sedimenti mmamịrị) |
| Usoro ule | Teknụzụ PCR nke fluorescence quantitative |
| Ụdị ọdabara | AB7500 |
| Nkọwa nkwakọ ngwaahịa | 48 ule / ngwa |
| Ọnọdụ Nchekwa | Ekwesịrị ịchekwa Kit A na 2-30 ℃ Ekwesịrị ịchekwa Kit B na -20± 5℃ Ọ bara uru ruo ọnwa iri na abụọ. |
