TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa cervical / Endometrial Cancer
NDỤMỌDỤ NKE EKPERE
Na-adịghị emerụ ahụ
Adabara ya na brush cervical na sample Pap smear.
Dị mma
Teknụzụ nchọpụta mbụ Me-qPCR methylation nwere ike mechaa n'otu nzọụkwụ n'ime awa 3 na-enweghị mgbanwe bisulfite.
N'oge mbụ
Achọpụtara ya n'oge mmalite nke ọrịa kansa.
Akpaaka
Soro ngwanrọ nyocha nsonaazụ ahaziri ahazi, nkọwa nke nsonaazụ ya na-akpaghị aka na enwere ike ịgụ ya ozugbo.
Ngwa ndapụta
Nyocha mbụ
Ndị nwere ahụike
Ntụle Ihe ize ndụ Ọrịa Cancer
Ọnụ ọgụgụ ndị nwere nnukwu ihe ize ndụ (nke dị mma maka papillomavirus mmadụ dị elu (hrHPV) ma ọ bụ ihe dị mma maka cytology exfoliation cervical / mma maka papillomavirus mmadụ dị elu (hrHPV) ma ọ bụ ihe dị mma maka cytology exfoliation cervical)
Nleba anya ugboro ugboro
Ọnụọgụ ndị amụma
Ezubere iji
A na-eji ngwa a maka nchọpụta qualitative in vitro hypermethylation nke mkpụrụ ndụ PCDHGB7 n'ụdị cervical.Maka ọrịa cancer cervical, nsonaazụ dị mma na-egosi mmụba dị elu nke ọkwa 2 ma ọ bụ ọkwa dị elu / ọkwa elu cervical intraepithelial neoplasia (CIN2 +, gụnyere CIN2, CIN3, adenocarcinoma in situ, na cancer cervical), nke chọrọ nyocha ọzọ nke colposcopy na / ma ọ bụ nyocha akụkọ ihe mere eme. .N'ụzọ megidere nke ahụ, nsonaazụ ule na-adịghị mma na-egosi na ihe ize ndụ nke CIN2 + dị ala, mana enweghị ike iwepụ ihe ize ndụ ahụ kpamkpam.Nchọpụta ikpeazụ kwesịrị ịdabere na colposcopy na / ma ọ bụ nsonaazụ histopathological.Ọzọkwa, maka ọrịa cancer endometrial, nsonaazụ dị mma na-egosi mmụba dị ukwuu nke ọnya precancerous endometrial na ọrịa kansa, nke chọrọ nyocha ọzọ nke akụkọ ihe mere eme nke endometrium.N'ụzọ megidere nke ahụ, nsonaazụ ule na-adịghị mma na-egosi na ihe ize ndụ nke ọnya precancerous endometrial na ọrịa cancer dị ala, mana enweghị ike iwepụ ihe ize ndụ ahụ kpamkpam.Nchọpụta ikpeazụ kwesịrị ịdabere na nsonaazụ histopathological nke endometrium.
PCDHGB7 bụ otu ezinụlọ protocadherin γ gene ụyọkọ.Achọpụtala Protocadherin ka ọ na-ahazi usoro ihe ndị dị ndụ dị ka mmụba cell, okirikiri cell, apoptosis, mbuso agha, mbugharị na autophagy nke mkpụrụ ndụ tumo site n'ụzọ dị iche iche na-egosi, na nkwụsị nke mkpụrụ ndụ ihe nketa nke hypermethylation nke mpaghara mgbasa ozi nwere njikọ chiri anya na ihe omume na mmepe. nke ọtụtụ ọrịa cancer.A kọwapụtara na hypermethylation nke PCDHGB7 jikọtara ya na etuto dị iche iche, dị ka lymphoma na-abụghị Hodgkin, ọrịa ara ara, cancer cervical, ọrịa cancer endometrial na ọrịa kansa eriri afo.
Ụkpụrụ Nchọpụta
Ngwa a nwere nucleic acid mmịpụta reagent na PCR reagent nchọpụta.A na-ewepụta acid nucleic site na usoro nke dabere na bead magnet.Ngwa a dabere na ụkpụrụ nke fluorescence quantitative PCR usoro, na-eji methylation-kpọmkwem ozugbo PCR mmeghachi omume iji nyochaa DNA ndebiri, na n'out oge chọpụta saịtị CpG nke PCDHGB7 gene na àgwà akara akara n'ime esịtidem ntụaka gene iberibe G1 na G2.A na-agbakọ ọkwa methylation nke PCDHGB7 na nlele, ma ọ bụ uru Me, dabere na uru PCDHGB7 gene methylated DNA amplification Ct na uru Ct nke ntụaka ahụ.A na-ekpebi PCDHGB7 gene hypermethylation nti ma ọ bụ adịghị mma dịka uru m siri dị.
