TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa endometrial
NDỤMỌDỤ NKE EKPERE
Nkenkenke
Ekwenyere ihe karịrị ụdị ụlọ ọgwụ 800 n'ime ọmụmụ ihe etiti iri abụọ kpuru isi, ngwaahịa a nwere nkọwapụta nke 82.81% yana nghọta nke 80.65%.
Dị mma
Teknụzụ nchọpụta mbụ Me-qPCR methylation nwere ike mechaa n'otu nzọụkwụ n'ime awa 3 na-enweghị mgbanwe bisulfite.
N'oge mbụ
Achọpụtara ya n'oge mmalite nke ọrịa kansa.
Akpaaka
Adabara ya na brush cervical na sample Pap smear.
Ezubere iji
A na-eji ngwa a maka nchọpụta qualitative in vitro nke hypermethylation nke mkpụrụ ndụ PCDHGB7 incervical specimens.Nsonaazụ dị mma na-egosi mmụba dị ukwuu nke ọnya precancerous endometrial na ọrịa kansa, nke chọrọ nyocha ọzọ nke akụkọ ihe mere eme nke endometrium.N'ụzọ megidere nke ahụ, nsonaazụ ule na-adịghị mma na-egosi na ihe ize ndụ nke ọnya precancerous endometrial na ọrịa cancer dị ala, mana enweghị ike iwepụ ihe ize ndụ ahụ kpamkpam.Nchọpụta ikpeazụ kwesịrị ịdabere na nsonaazụ histopathological nke endometrium.PCDHGB7 bụ otu ezinụlọ protocadherin γ gene ụyọkọ.Achọpụtala Protocadherin ka ọ na-ahazi usoro ihe ndị dị ndụ dị ka mmụba cell, okirikiri cell, apoptosis, mbuso agha, mbugharị na autophagy nke mkpụrụ ndụ tumo site n'ụzọ dị iche iche na-egosi, na nkwụsị nke mkpụrụ ndụ ihe nketa nke hypermethylation nke mpaghara mgbasa ozi nwere njikọ chiri anya na ihe omume na mmepe. nke ọtụtụ ọrịa cancer.A kọwapụtara na hypermethylation nke PCDHGB7 jikọtara ya na etuto dị iche iche, dị ka lymphoma na-abụghị Hodgkin, ọrịa ara ara, cancer cervical, ọrịa cancer endometrial na ọrịa kansa eriri afo.
Ụkpụrụ Nchọpụta
Ngwa a nwere nucleic acid mmịpụta reagent na PCR reagent nchọpụta.A na-ewepụta acid nucleic site na usoro nke dabere na bead magnet.Ngwa a dabere na ụkpụrụ nke fluorescence quantitative PCR usoro, na-eji methylation-kpọmkwem ozugbo PCR mmeghachi omume iji nyochaa DNA ndebiri, na n'out oge chọpụta saịtị CpG nke PCDHGB7 gene na àgwà akara akara n'ime esịtidem ntụaka gene iberibe G1 na G2.A na-agbakọ ọkwa methylation nke PCDHGB7 na nlele, ma ọ bụ uru Me, dabere na uru PCDHGB7 gene methylated DNA amplification Ct na uru Ct nke ntụaka ahụ.A na-ekpebi PCDHGB7 gene hypermethylation nti ma ọ bụ adịghị mma dịka uru m siri dị.
Ngwa ndapụta
Nyocha mbụ
Ndị nwere ahụike
Ntụle Ihe ize ndụ Ọrịa Cancer
Otu ndị nwere nnukwu ihe ize ndụ (ndị nwere ọbara ọgbụgba na-adịghị mma mgbe ha menopause, endometrial thickening, wdg).
Nleba anya ugboro ugboro
Ọnụọgụ ndị amụma
Mkpa ụlọ ọgwụ
Nyocha mbụ maka ndị mmadụ nwere ahụike:Enwere ike ihichapụ ọrịa cancer endometrial na ọnya ndị na-ebute ụzọ nke ọma;
Ntụle ihe egwu maka ndị nwere nnukwu ihe ize ndụ:Enwere ike ịme nyocha ihe ize ndụ maka ndị nwere ọbara ọgbụgba na-adịghị mma na nke endometrial mgbe menopause gasịrị iji nyere aka na nyocha ụlọ ọgwụ;
Nleba anya nlọghachite nke ndị mmadụ:Enwere ike ileba anya nleba anya nlọghachi nke ndị mmadụ mgbe arụchara ọrụ gasịrị iji gbochie igbu oge na ọgwụgwọ nke nlọghachite kpatara.
Nchịkọta ihe atụ
Usoro nlele: Debe ihe nlele cervical a na-atụfu na cervical os, jiri nwayọọ na-ete ahịhịa cervical wee tụgharịa ugboro 4-5 n'akụkụ elekere, jiri nwayọọ wepụ ahịhịa cervical, tinye ya na ngwọta nchekwa cell, ma kpọọ ya maka nyocha na-esonụ.
Nchekwa sample:Enwere ike ịchekwa ihe nlele na ụlọ okpomọkụ ruo ụbọchị 14, na 2-8 ℃ ruo ọnwa 2, yana na -20 ± 5 ℃ ruo ọnwa 24.
Usoro nchọpụta: awa 3 (na-enweghị usoro ntuziaka)
TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa endometrial
| Ngwa ụlọ ọgwụ | Nchọpụta ihe enyemaka Clinical nke endometrial carcinoma |
| Nchọpụta mkpụrụ ndụ ihe nketa | PCDHGB7 |
| Ụdị ihe atụ | Ụdị cervical nwanyị |
| Usoro ule | Teknụzụ PCR nke fluorescence quantitative |
| Ụdị ọdabara | AB7500 |
| Nkọwa nkwakọ ngwaahịa | 48 ule / ngwa |
| Ọnọdụ Nchekwa | Ekwesịrị ịchekwa Kit A na 2-30 ℃ Ekwesịrị ịchekwa Kit B na -20± 5℃ Ọ bara uru ruo ọnwa iri na abụọ. |
Gbasara anyị
Epiprobe nwere a keukwu akụrụngwa ewu: GMP mmepụta center na-ekpuchi ebe 2200 square mita, na-ejigide ISO13485 àgwà management usoro, nke na-ezute mmepụta chọrọ nke ụdị nile nke mkpụrụ ndụ ihe nketa ule reagent ngwaahịa;ụlọ nyocha ahụike na-ekpuchi mpaghara 5400 square mita ma nwee ikike ịme azụmahịa nchọpụta ọrịa cancer methylation dị ka ụlọ nyocha ahụike nke ndị ọzọ gbaara agbazi.Na mgbakwunye, anyị nwere ngwaahịa atọ enwetara asambodo CE, na-ekpuchi ọrịa kansa cervical, ọrịa kansa endometrial na ọrịa kansa urothelial metụtara.
Enwere ike iji teknụzụ nchọpụta mkpụrụ ndụ cancer nke Epiprobe maka nyocha ọrịa kansa n'oge, nchọpụta inyeaka, nyocha tupu ịwa ahụ na mgbe emechara ya, nlekota oru nyocha, nke na-aga n'ihu na usoro nyocha na ọgwụgwọ ọrịa cancer dum, na-enye ngwọta dị mma maka ndị dọkịta na ndị ọrịa.
