TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa urothelial
NDỤMỌDỤ NKE EKPERE
Nkenkenke
Ekwenyere ihe karịrị ihe nlele ụlọ ọgwụ 3500 n'ime ọmụmụ ihe n'ihu ọha, ngwaahịa a nwere nkọwapụta nke 92.7% yana nghọta nke 82.1%.
Dị mma
Teknụzụ nchọpụta mbụ Me-qPCR methylation nwere ike mechaa n'otu nzọụkwụ n'ime awa 3 na-enweghị mgbanwe bisulfite.
Na-adịghị emerụ ahụ
Naanị 30 ml nke ihe nlele mmamịrị ka achọrọ iji chọpụta ụdị ọrịa kansa atọ, gụnyere ọrịa kansa pelvis, ọrịa urethra, kansa eriri afọ n'otu oge.
Ngwa ndapụta
Nchọpụta ihe enyemaka: Enwere ike nyochaa ndị ọrịa nwere ọrịa kansa urothelial n'ụzọ na-adịghị mma iji nyere aka na nyocha ụlọ ọgwụ.
Ntụle arụmọrụ ịwa ahụ / chemotherapy: Nyochaa ịdị irè nke ịwa ahụ / chemotherapy iji nyere aka melite ahụike nke mmetụta ọgwụgwọ.
Nleba anya nlọghachi nke ndị mmadụ mgbe arụchara ọrụ gasịrị:Enwere ike nyochaa ndị ọrịa nwere ọrịa cancer urothelial maka nlọghachi azụ n'ụzọ na-adịghị mma na-adịghị mma, nke na-eme ka nnabata ndị ọrịa dịkwuo mma.
Nchịkọta ihe atụ
Usoro nlele: Usoro nlele: Chịkọta ihe nlele mmamịrị (mmamịrị ụtụtụ ma ọ bụ mmamịrị na-enweghị usoro), tinye ihe nchekwa mmamịrị wee gwakọta nke ọma, chekwaa ya n'ime ụlọ ma kpọọ ya maka nyocha a.
Nchekwa sample: Enwere ike ịchekwa ihe nlele na ụlọ okpomọkụ ruo ụbọchị 14, na 2-8 ℃ ruo ọnwa 2, yana na -20 ± 5 ℃ ruo ọnwa 24.
Usoro nchọpụta: awa 3 (na-enweghị usoro ntuziaka)
Ngwa nyocha Methylation DNA (qPCR) maka ọrịa kansa urothelial
| Ngwa ụlọ ọgwụ | Nchọpụta ihe enyemaka ụlọ ọgwụ nke ọrịa cancer urothelial;nyocha arụmọrụ ịwa ahụ / chemotheray;nleba anya nlọghachi azụ nke arụ ọrụ |
| Nchọpụta mkpụrụ ndụ ihe nketa | UC |
| Ụdị ihe atụ | Urine exfoliated cell sample (sedimenti mmamịrị) |
| Usoro ule | Teknụzụ PCR nke fluorescence quantitative |
| Ụdị ọdabara | AB7500 |
| Nkọwa nkwakọ ngwaahịa | 48 ule / ngwa |
| Ọnọdụ Nchekwa | Ekwesịrị ịchekwa Kit A na 2-30 ℃Ekwesịrị ịchekwa Kit B na -20± 5℃ Ọ bara uru ruo ọnwa iri na abụọ. |
