TAGMe DNA Methylation Kits (qPCR) maka ọrịa kansa cervical
NDỤMỌDỤ NKE EKPERE
Nkenkenke
Akwadoro n'elu ihe nlele ụlọ ọgwụ 36000 n'ime ọmụmụ ihe iri abụọ na-ekpuchi anya, ngwaahịa a nwere nkọwapụta nke 94.3% yana mmetụta nke 96.0%.
Dị mma
Teknụzụ nchọpụta mbụ Me-qPCR methylation nwere ike mechaa n'otu nzọụkwụ n'ime awa 3 na-enweghị mgbanwe bisulfite.
N'oge mbụ
Enwere ike ime nyocha ọrịa kansa cervical ruo n'ọkwa nke ọnya dị elu (ọnya buru ụzọ).
Akpaaka
Soro ngwanrọ nyocha nsonaazụ ahaziri ahazi, nkọwa nke nsonaazụ ya na-akpaghị aka na enwere ike ịgụ ya ozugbo.
Ezubere iji
A na-eji ngwa a maka nchọpụta qualitative in vitro hypermethylation nke mkpụrụ ndụ PCDHGB7 n'ụdị cervical.Nsonaazụ dị mma na-egosi mmụba dị elu nke ọkwa 2 ma ọ bụ ọkwa dị elu / ọkwa dị elu nke cervical intraepithelial neoplasia (CIN2+, gụnyere CIN2, CIN3, adenocarcinoma in situ, na cervical cancer), nke na-achọkwu nyocha nke colposcopy na / ma ọ bụ histopathological.N'ụzọ megidere nke ahụ, nsonaazụ ule na-adịghị mma na-egosi na ihe ize ndụ nke CIN2 + dị ala, mana enweghị ike iwepụ ihe ize ndụ ahụ kpamkpam.Nchọpụta ikpeazụ kwesịrị ịdabere na colposcopy na / ma ọ bụ nsonaazụ histopathological.PCDHGB7 bụ otu ezinụlọ protocadherin γ gene ụyọkọ.Achọpụtala Protocadherin ka ọ na-ahazi usoro ihe ndị dị ndụ dị ka mmụba cell, okirikiri cell, apoptosis, mbuso agha, mbugharị na autophagy nke mkpụrụ ndụ tumo site n'ụzọ dị iche iche na-egosi, na nkwụsị nke mkpụrụ ndụ ihe nketa nke hypermethylation nke mpaghara mgbasa ozi nwere njikọ chiri anya na ihe omume na mmepe. nke ọtụtụ ọrịa cancer.A kọwapụtara na hypermethylation nke PCDHGB7 jikọtara ya na etuto dị iche iche, dị ka lymphoma na-abụghị Hodgkin, ọrịa ara ara, cancer cervical, ọrịa cancer endometrial na ọrịa kansa eriri afo.
Ụkpụrụ Nchọpụta
Ngwa a nwere nucleic acid mmịpụta reagent na PCR reagent nchọpụta.A na-ewepụta acid nucleic site na usoro nke dabere na bead magnet.Ngwa a dabere na ụkpụrụ nke fluorescence quantitative PCR usoro, na-eji methylation-kpọmkwem ozugbo PCR mmeghachi omume iji nyochaa DNA ndebiri, na n'out oge chọpụta saịtị CpG nke PCDHGB7 gene na àgwà akara akara n'ime esịtidem ntụaka gene iberibe G1 na G2.A na-agbakọ ọkwa methylation nke PCDHGB7 na nlele, ma ọ bụ uru Me, dabere na uru PCDHGB7 gene methylated DNA amplification Ct na uru Ct nke ntụaka ahụ.A na-ekpebi PCDHGB7 gene hypermethylation nti ma ọ bụ adịghị mma dịka uru m siri dị.
Ngwa ndapụta
Nyocha mbụ
Ndị nwere ahụike
Ntụle Ihe ize ndụ Ọrịa Cancer
Ọnụ ọgụgụ ndị nwere nnukwu ihe ize ndụ (nke dị mma maka papillomavirus mmadụ nwere nnukwu ihe ize ndụ (hrHPV) ma ọ bụ ihe dị mma maka cytology exfoliation cervical)
Nleba anya ugboro ugboro
Ọnụọgụ ndị mmadụ mechara arụ ọrụ (nke nwere akụkọ ihe mere eme nke ọnya cervical dị elu ma ọ bụ kansa cervical)
Mkpa ụlọ ọgwụ
Nyocha mbụ maka ndị mmadụ nwere ahụike:Enwere ike ihichapụ ọrịa kansa cervical na ọnya ndị nwere ọrịa kansa nke ọma
Ntụle ihe ize ndụ na ọnụ ọgụgụ ndị nwere nnukwu ihe ize ndụ:Enwere ike ịme nhazi ọkwa ihe egwu na ndị nwere HPV iji duzie nchọpụta nhụta na-esote
Nleba anya ugboro ugboro maka ọnụ ọgụgụ ndị bi n'oge a na-arụ ọrụ:Enwere ike ileba anya nleba anya nlọghachi nke ndị mmadụ mgbe arụchara ọrụ gasịrị iji gbochie igbu oge na ọgwụgwọ nke nlọghachite kpatara
Nchịkọta ihe atụ
Usoro nlele: Debe ihe nlele cervical a na-atụfu na cervical os, jiri nwayọọ na-ete ahịhịa cervical wee tụgharịa ugboro 4-5 n'akụkụ elekere, jiri nwayọọ wepụ ahịhịa cervical, tinye ya na ngwọta nchekwa cell, ma kpọọ ya maka nyocha na-esonụ.
Nchekwa sample:Enwere ike ịchekwa ihe nlele na ụlọ okpomọkụ ruo ụbọchị 14, na 2-8 ℃ ruo ọnwa 2, yana na -20 ± 5 ℃ ruo ọnwa 24.
Usoro nchọpụta: awa 3 (na-enweghị usoro ntuziaka)
Ngwa nyocha Methylation DNA (qPCR) maka ọrịa kansa urothelial
| Ngwa ụlọ ọgwụ | Nchọpụta ihe enyemaka Clinical nke ọrịa kansa cervical |
| Nchọpụta mkpụrụ ndụ ihe nketa | PCDHGB7 |
| Ụdị ihe atụ | Ụdị cervical nwanyị |
| Usoro ule | Teknụzụ PCR nke fluorescence quantitative |
| Ụdị ọdabara | AB7500 |
| Nkọwa nkwakọ ngwaahịa | 48 ule / ngwa |
| Ọnọdụ Nchekwa | Ekwesịrị ịchekwa Kit A na 2-30 ℃ Ekwesịrị ịchekwa Kit B na -20± 5℃ Ọ bara uru ruo ọnwa iri na abụọ |
