Gargle Nucleic Acid Reagents Mwepụta
Ụkpụrụ Nchọpụta
Ngwaahịa a na-enwekarị gargle, ngwakọta superparamagnetic nanospheres na-amị amị, yana reagents pụrụ iche.Mpempe akwụkwọ ndọta pụrụ iche agbakwunyere nwere ezigbo mmekọrịta maka akụrụngwa anụ ahụ (gụnyere nje na-enweghị nje na mkpụrụ ndụ nje butere).Mgbe ị na-akpọtụrụ ngwọta lysis, ndị na-abụghị ionic cell / nucleus-membrane-breaking surfactants na protease inhibitors na ngwọta nwere ike igbochi ọrụ nke DNA / RNA enzyme, ma mee ka nucleic acid guzosie ike.A na-ewepụta ihe niile nucleic acid dị na akụkụ anụ ahụ nke gargle nke ọma na ngwọta lysis, na-enweta ngwa ngwa nucleic acid.Ihe nlele a na-eji ngwa a anaghị achọ nchacha acid nucleic, nke nwere ike itinye aka ozugbo na ngwa nchọpụta nucleic acid.
Isi ihe nke reagent
E gosipụtara akụkụ ndị ahụ na tebụl 1.
Tebụl 1 Ngwa na ibunye na Kit
| Aha akụrụngwa | Isi ihe | Nha (1) | Nha (10) | Nha (30) | Nha (50) |
| 1. Gari A | NaCl | 8 ml / tube | 8ml / tube * 10 ọkpọkọ | 8ml / tube * 30 ọkpọkọ | 8ml / tube * 50 ọkpọkọ |
| 2. Onye na-anakọta ahịhịa | PP | 1 ibe | 10 pcs | 30 pcs | 50 pcs |
| 3. Ngwọta Ọganihu B | Igwe igwe magnetik | 2 ml / tube | 2ml / tube * 10 akpa | 2ml / tube * 30 ọkpọkọ | 2ml / tube * 50 tubes |
| 4 Lysis Buffer C | Protease K | 0.2ml / iberibe | 0.2ml / iberibe * 10 pcs | 0.2ml / iberibe * 30 pcs | 0.2ml / iberibe * 50 pcs |
| 5. Igwe Igwe Ọdụdọ | Igwe Ọdụdọ | 1 ibe | 10 pcs | 30 pcs | 50 pcs |
Akụkụ ndị ahụ dị irè maka ọnwa 12.
Ngwa ndị achọrọ na mmịpụta nucleic acid, mana etinyeghị ya na ngwa:
1. Ihe eji eme ihe: 1.5ml EP tube;
2. Akụrụngwa: Mmiri ịsa ahụ (ma ọ bụ metal bath), pipettes, na centrifuge.
Ozi bụ isi
Ihe atụ chọrọ:
1. Ọdabara na nucleic acid mmịpụta na ịba ụba nke gargle samples.
2. A ga-agbakwunye ihe nlele gargle na ngwọta nkwalite B na oge mgbe nchịkọta.A ga-ebufe bead magnetik ndị ewepụtara na ebe nchekwa lysis C ozugbo.Enwere ike ịchekwa ihe nlele agbakwunyere na ebe nchekwa lysis C na okpomọkụ ụlọ.
Nkọwa nkwakọ ngwaahịa: 1 ibe / igbe, 10 pcs / igbe, 30 pcs / igbe, na 50 pcs / igbe.
Ọnọdụ nchekwa: The enrichment ngwọta B na lysis ngwọta C kwesịrị ịchekwa ọnwa 12 na 2-8 ℃, na ihe ndị ọzọ nwere ike ịchekwa maka ọnwa 12 n'okpuru r ụlọ okpomọkụ;Enwere ike ibugharị ngwa ahụ ma chekwaa ya nwa oge n'okpuru ọnọdụ okpomọkụ, nke agaghị agafe ụbọchị 5.
Oge nkwado: Ọnwa iri na abụọ
Asambodo ndekọ ngwaọrụ ahụike Mba/ngwaahịa chọrọ teknụzụ Mba.:HJXB Nọmba 20220086.
Ụbọchị nnabata na ntughari ntuziaka ndị a:
Ụbọchị nkwado: Ọkt 26, 2022
