Ngwa Nwepụta Acid Nucleic (A02)
Ụkpụrụ nchọpụta
Mgbe ahapụchara DNA genomic site na kewaa mkpụrụ ndụ na ihe nchekwa lysis, bead magnet nwere ike họrọ ijikọ na DNA genomic dị na nlele.Enwere ike iwepụ ọnụ ọgụgụ dị nta nke adịghị ọcha nke eriri magnetik na-etinye n'ime ya site na ihe nchekwa ihe.Na TE, bead magnetik nwere ike ịhapụ DNA boundgenome, nweta DNA genome dị elu.Usoro a dị mfe na ngwa ngwa na àgwà DNA amịpụtara dị elu, nke nwere ike izute ihe achọrọ maka nchọpụta DNA methylation.Ka ọ dị ugbu a, ngwa mmịpụta nke dabere na bead magnet nwere ike dakọtara na mmịpụta nucleic acid na-akpaghị aka, na-ezute ọrụ mmịpụta nucleic acid dị elu.
Isi ihe nke reagent
E gosipụtara akụkụ ndị a na tebụl 1:
Tebụl 1 Ngwa Reagent na Nbudata
| Aha akụrụngwa | Isi ihe | Nha (48) | Nha (200) |
| 1. Ihe nchekwa mgbaze A | Tris, SDS | 15.8 ml / karama | 66 ml / karama |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 ml / karama | 66 ml / karama |
| 3. Ihe nchekwa A | NaCl, Tris | 11 ml / karama | 44 ml / karama |
| 4. Ebe nchekwa B | NaCl, Tris | 13 ml / karama | 26.5mL / karama * 2 |
| 5. TE | Tris, EDTA | 12 ml / karama | 44 ml / karama |
| 6. Protease K ngwọta | Protease K | 1.1ml / iberibe | 4.4ml / iberibe |
| 7. Nkwusioru bead magnetik 2 | Igwe igwe magnetik | 0.5ml / iberibe | 2.2ml / iberibe |
| 8. Ntuziaka iji wepụ nucleic acid reagents | / | 1 oyiri | 1 oyiri |
Ngwa ndị achọrọ na mmịpụta nucleic acid, mana etinyeghị ya na ngwa:
1. Reagent: ethanol anhydrous, isopropanol, na PBS;
2. Ihe eji eme ihe: 50mL centrifuge tube na 1.5mL EP tube;
3. Akụrụngwa: Mmiri ịsa ahụ, pipettes , magnetik shelf, centrifuge, 96-well efere (akpaka), akpaka nucleic acid mmịpụta akụrụngwa (akpaka).
Ozi bụ isi
Ihe atụ chọrọ:
1. A ga-emecha nchọpụta ahụ n'okpuru nchekwa ụbọchị 7 nke ọnọdụ okpomọkụ mgbe nchịkọta nke cervical exfoliated cell sample (anaghị edozi).
2. A ga-emecha nchọpụta ahụ n'okpuru nchekwa ụbọchị 30 nke ọnọdụ okpomọkụ mgbe nchịkọta nke cervical exfoliated cell sample (obere).
3. A ga-emecha nchọpụta ahụ n'okpuru nchekwa ụbọchị 30 nke ọnọdụ okpomọkụ mgbe nchịkọta nke mmamịrị mmamịrị;A ga-emecha nchọta ahụ n'oge na-achịkọta ihe nlele cell omenala.
Nkọwa ụgbọ ala:200 pcs / igbe, 48 pcs / igbe.
Ọnọdụ nchekwa:2-30 ℃
Oge nkwado:Ọnwa iri na abụọ
Ngwa ọdabara:Tianlong NP968-C nucleic acid mmịpụta ngwá ọrụ, Tiangen TGuide S96 nucleic acid mmịpụta ngwá ọrụ, GENE DIAN EB-1000 nucleic acid mmịpụta ngwá.
Asambodo ndekọ ngwaọrụ ahụike Mba/ngwaahịa chọrọ teknụzụ Mba.:HJXB Nọmba 20210100.
Ụbọchị nkwado na ntughari ntuziaka:Ụbọchị nkwado: Nov. 18, 2021
Gbasara anyị
Dị ka ụlọ ọrụ teknụzụ dị elu tọrọ ntọala na 2018 site n'aka ndị ọkachamara epigenetic kachasị elu, Epiprobe na-elekwasị anya na nchọpụta mkpụrụ ndụ nke ọrịa cancer DNA methylation na ụlọ ọrụ ọgwụgwọ ziri ezi.Site na ntọala teknụzụ dị omimi, anyị bu n'obi iduga oge nke ngwaahịa ọhụrụ iji nweta ọrịa kansa na nwa osisi!
Dabere na nyocha ogologo oge nke Epiprobe isi otu, mmepe na mgbanwe n'ọhịa nke DNA methylation na mbelata ọnụ ọhụrụ, jikọtara ya na ihe pụrụ iche DNA methylation lekwasịrị anya nke ọrịa cancer, anyị na-eji a pụrụ iche multivariate algọridim na-ejikọta nnukwu data na nkà na ụzụ ọgụgụ isi. n'onwe ya wepụta teknụzụ biopsy mmiri mmiri echekwara puru iche.Site n'ịtụle ọkwa methylation nke saịtị ụfọdụ nke iberibe DNA n'efu na nlele ahụ, a na-ezere adịghị ike nke ụzọ nyocha ọdịnala na njedebe nke ịwa ahụ na ịwa ahụ, nke na-abụghị naanị na-enweta nchọpụta ziri ezi nke ọrịa cancer mbụ, kamakwa na-enyere aka nyochaa oge. nke ọrịa cancer na mmepe mmepe.
